Turbulent Dynamics of Epithelial Cell Cultures

Turbulent dynamics of epithelial cell cultures

In vitro epithelial monolayers exhibit a rich repertoire of collective dynamical behaviours according to microenvironment mechanical features. We investigate the statistical and dynamical properties of human bronchial epithelial cell (HBEC) flows in bulk, by analysing their large length-scale and long time-scale spatiotemporal dynamics. Activity-driven spontaneous collective flows consist of an...

[Expansion of primary bronchial epithelial cell cultures].

OBJECTIVE Cell cultures provide a good model for studying lung diseases but they are difficult to reproduce and the number of cells obtained is limited. The aim of this study was to develop a way to increase the production of human bronchial epithelial cells (BEC) in primary cultures. MATERIAL AND METHODS A total of 12 samples (9 from surgical specimens and 3 from endoscopic biopsies) were pr...

Well-differentiated human airway epithelial cell cultures.

Mucociliary interactions and mucus dynamics in ciliated human bronchial epithelial cell cultures.

The airway epithelial surface liquid is generally considered to be composed of two layers, a periciliary layer and a continuous thick mucus layer moving in bulk. This view may not be appropriate for all areas of the lung. Our hypothesis, that mucus may form a discontinuous layer with dynamic attachments to the surface, is investigated using a culture system. We used live-cell confocal microscop...

Airway Epithelial Cell Migration Dynamics

Cell spreading and migration associated with the expression of the 92-kD gelatinase (matrix metalloproteinase 9 or MMP-9) are important mechanisms involved in the repair of the respiratory epithelium. We investigated the location of MMP-9 and its potential role in migrating human bronchial epithelial cells (HBEC). In vivo and in vitro, MMP-9 accumulated in migrating HBEC located at the leading ...